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Estimated Number of Cells

4,186

Post-Normalization Mean Reads per Cell

79,583

Median Genes per Cell

1,815
Pre-Normalization Number of Reads
Total number of read pairs that were assigned to these libraries in demultiplexing.
Post-Normalization Number of Reads
Number of read pairs after normalizing for depth among multiple libraries.

Sequencing

Pre-Normalization Number of Reads 364,006,438
Post-Normalization Number of Reads 333,136,686
library 0 Fraction of Reads Kept
The fraction of reads kept for this input sample, after normalizing depth across samples to reduce batch effects.
library 1 Fraction of Reads Kept
The fraction of reads kept for this input sample, after normalizing depth across samples to reduce batch effects.
library 0 Pre-Normalization Total Reads per Cell
The mean number of total sequencing reads per cell in this input sample, prior to depth normalization.
library 1 Pre-Normalization Total Reads per Cell
The mean number of total sequencing reads per cell in this input sample, prior to depth normalization.
library 0 Pre-Normalization Confidently Mapped Barcoded Reads per Cell
The mean number of confidently-mapped-to-transcriptome, valid-barcode reads per cell in this input sample, prior to depth normalization.
library 1 Pre-Normalization Confidently Mapped Barcoded Reads per Cell
The mean number of confidently-mapped-to-transcriptome, valid-barcode reads per cell in this input sample, prior to depth normalization.

Aggregation

library 0 Fraction of Reads Kept 100.0%
library 1 Fraction of Reads Kept 82.7%
library 0 Pre-Normalization Total Reads per Cell 78,870
library 1 Pre-Normalization Total Reads per Cell 97,330
library 0 Pre-Normalization Confidently Mapped Barcoded Reads per Cell 53,200
library 1 Pre-Normalization Confidently Mapped Barcoded Reads per Cell 64,324
 
Estimated Number of Cells
The total number of barcodes associated with cell-containing partitions, estimated from the barcode count distribution.
Fraction Reads in Cells
The fraction of valid-barcode, confidently-mapped-to-transcriptome reads with cell-associated barcodes.
Pre-Normalization Mean Reads per Cell
The total number of sequenced reads divided by the number of barcodes associated with cell-containing partitions.
Post-Normalization Mean Reads per Cell
The total number of (depth-normalized) reads divided by the number of barcodes associated with cell-containing partitions.
Median Genes per Cell
The median number of genes detected per cell-associated barcode. Detection is defined as the presence of at least 1 UMI count.
Median UMI Counts per Cell
The median number of UMI counts per cell-associated barcode.

Cells

Estimated Number of Cells 4,186
Fraction Reads in Cells 94.4%
Pre-Normalization Mean Reads per Cell 86,958
Post-Normalization Mean Reads per Cell 79,583
Median Genes per Cell 1,815
Median UMI Counts per Cell 5,934

Sample

Name JP32JP33
Description
Transcriptome mm10
Chemistry Single Cell 3' v2
Cell Ranger Version 2.2.0

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